Heather J. Lacey, Rachel Chen, Daniel Vuong, Ernest Lacey, Peter J. Rutledge, Yit-Heng Chooi, Andrew M. Piggott and Thomas J. Booth
Organic and Biomolecular Chemistry 21, 2531-2538.
Publication Date: Feb 27, 2023
https://doi.org/10.1039/D2OB02332F
Abstract:
Fourteen-membered macrolides are a class of compounds with significant clinical value as antibacterial agents. As part of our ongoing investigation into the metabolites of Streptomyces sp. MST-91080, we report the discovery of resorculins A and B, unprecedented 3,5-dihydroxybenzoic acid (α-resorcylic acid)-containing 14-membered macrolides. We sequenced the genome of MST-91080 and identified the putative resorculin biosynthetic gene cluster (rsn BGC). The rsn BGC is hybrid of type I and type III polyketide synthases. Bioinformatic analysis revealed that the resorculins are relatives of known hybrid polyketides: kendomycin and venemycin. Resorculin A exhibited antibacterial activity against Bacillus subtilis (MIC 19.8 μg mL−1), while resorculin B showed cytotoxic activity against the NS-1 mouse myeloma cell line (IC50 3.6 μg mL−1).
